02089nas a2200205   4500008003900000245006300039210006000102260002000162520144900182653001901631653001601650653002401666100001501690700001801705700001701723700001701740700001401757700001601771856009601787       2012                          d00aA multi-stimuli environment for cardiac tissue engineering0 amultistimuli environment for cardiac tissue engineering  aVienna, Austria3 a<p>The identification of the ideal cell source to generate cardiac tissue able to integrate into the host myocardium and with the contractile system is crucial for cardiac engineering. Amongst different cell sources so far proposed, human adult Cardiac Progenitor Cells (hCPCs) show the ability to proliferate and differentiate toward cardiac lineages when grown in appropriate microenvironmental conditions. It is widely accepted that conventional 2D cultures may provide a physiological environment for growing cells. For this reason the need to have an engineered microenvironment, matching physiological requirements, is crucial. A 3D context with spatial and time varying distribution of regulatory factors using mechanically matched scaffolds and bioreactors could represent an in vitro cell culture model being able to more closely reflects the in vivo conditions. In the present study, the possibility of using biocompatible and biodegradable scaffolds of collagen based or derivatives hydrogels in combination with Linneg/Sca-1pos hCPCs gathered from human heart biopsies was investigated. Bio-constructs were placed in the low shear, high flow MCmB (MultiCompartment modular Bioreactor) and the combined effects of dynamic culture conditions and 3D scaffolds on cell morphology and differentiation were studied in order to investigate the possibility of fabricating stem cell-derived cardiac patches to replace infarcted tissue.</p>
10aBioengineering10aBioreactors10aDynamic 3D Cultures1 aTirella, A1 aAhluwalia, A.1 aDi Nardo, P.1 aGaudiello, E1 aGiusti, S1 aPagliari, F  uhttp://95.110.227.47/publications/multi-stimuli-environment-cardiac-tissue-engineering.html01650nas a2200193   4500008004100000245009200041210006900133260000800202300001400210490000800224520099800232653001901230653001601249100001501265700002001280700001401300700001801314856012401332       2010                        eng d00aA low shear stress modular bioreactor for connected cell culture under high flow rates.0 alow shear stress modular bioreactor for connected cell culture u  cMay  a127–1370 v1063 a<p>A generic "system on a plate" modular multicompartmental bioreactor array which enables microwell protocols to be transferred directly to the bioreactor modules, without redesign of cell culture experiments or protocols is described. The modular bioreactors are simple to assemble and use and can be easily compared with standard controls since cell numbers and medium volumes are quite similar. Starting from fluid dynamic and mass transport considerations, a modular bioreactor chamber was first modeled and then fabricated using "milli-molding," a technique adapted from soft lithography. After confirming that the shear stress was extremely low in the system in the range of useful flow rates, the bioreactor chambers were tested using hepatocytes. The results show that the bioreactor chambers can increase or maintain cell viability and function when the flow rates are below 500 microL/min, corresponding to wall shear stresses of 10(-5) Pa or less at the cell culture surface.</p>
10aBioengineering10aBioreactors1 aMazzei, D.1 aGuzzardi, M. A.1 aGiusti, S1 aAhluwalia, A.  uhttp://95.110.227.47/publications/low-shear-stress-modular-bioreactor-connected-cell-culture-under-high-flow-rates.html